|
KMID : 0380620160480060590
|
|
Korean Journal of Food Science and Technology
2016 Volume.48 No. 6 p.590 ~ p.596
|
|
|
Antioxidant, anti-inflammatory, and anti-pruritic effects of grape branch extract
|
|
Cho Byoung-Ok
Yin Hong-Hua
Che Denis Nchang
Kim Sang-Jun
Ryu Cheol
Jang Seon-Il
|
|
|
Abstract
|
|
|
|
This study was designed to investigate the antioxidant activities of the ethanol extracts (GBE) of grape branches (Campbell Early). The total polyphenol and flavonoid content of GBE was 201.42±4.16 and 11.85±0.44 mg GAE/g, respectively. The antioxidant activity of GBE was measured using the ABTS and DPPH assays, and the IC50 values were 45.60±0.09 and 299.13±0.22, respectively. GBE inhibited the production of pro-inflammatory mediators (NO, iNOS, PGE2, COX-2, IL-1β, and IL-6) in lipopolysaccharide-stimulated RAW 264.7 macrophages in a dose-dependent manner. Moreover, GBE treatment significantly suppressed the production of TNF-α and IL-6 cytokines in phorbol 12- myristate 13-acetate plus calcium ionophore A23187-stimulated HMC-1 human mast cells. Furthermore, the administration of GBE markedly inhibited the scratching behavior induced by the compound 48/80 in ICR mice. These results suggested that GBE has potential as a therapeutic agent against inflammation and itch-related skin diseases.
|
|
|
KEYWORD
|
|
|
Grape branch, antioxidant, anti-inflammation, mast cells, scratching behavior
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|